The implementation of power gradients in both the xy- and z-directions via the Think3D software provides users with the capability to achieve diverse stiffness within their printed constructs. This functionality holds significant promise in cell culture applications, enabling researchers to mimic the intricate stiffness gradients present in biological tissues. Offering options such as linear, Gaussian, and gyroid gradients, users have extensive flexibility to explore and experiment with various stiffness profiles. This versatility empowers researchers to delve into the impact of varying mechanical properties on cell behavior, tissue development, and other biological processes, thereby providing invaluable insights into tissue engineering and regenerative medicine.